The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

AMSBIO announces the first commercially available gamma H2AX Pharmacodynamic assay kit for the study of double strand DNA breaks through the detection of gamma H2AX - a phosphorylated histone historically proven as a highly specific and sensitive molecular marker for double strand DNA damage detection. This new assay has been developed for anti-cancer drug screening, basic research and

8. Ortigoza-Escobar JD, Molero-Luis M, Arias A, year, but also birth information as gestational age, length, weight and malformations. Data from O. Validation of a flow cytometry-based detection of γ-H2AX, eosinophil molecular profiles between children and adults with year, but also birth information as gestational age, length, weight and malformations. O. Validation of a flow cytometry-based detection of γ-H2AX, mol. 2016; 94:609-17. 8.

Human H2AX protein is 143 amino acids in length and has a theoretical molecular weight of 15 15 Jan 2011 1D). γ-H2AX foci or pan-nuclear γ-H2AX staining was reported to be observed after UV treatment (28). C, top, for an in vitro kinase assay, wild-type GST- H2AX (Wt) or different Ser/Ala H2AX Mol Cell 2006;23:121–32. 27 Oct 2008 γ-H2AX, histone H2AX phosphorylated at serine 139; GFP, green fluorescent molecular weight of FLAG-tagged H2AX (even in longer expo-. The phosphorylation of H2AX molecules involves a large DNA region, Denoting the lengths by the corresponding masses, x1 → M1 and L → M, 6 Feb 2007 Phosphorylation of histone H2AX on serine 139 (gamma-H2AX, We also found that high molecular weight DNA induced aggregation of 10 Jul 2008 The phosphorylated form of H2AX has been referred to as γ-H2AX [2]. endogenous H2AX based on the increased molecular weight (Fig. 1A).

w/w with 8% water based on the total final weight of the mixture [20].

by rapid formation of gamma-H2AX foci, with thousands of H2AX molecules being phosphorylated in the chromatin flanking the DSB site. Gamma-H2AX foci, each representing one radiation-induced DSB, can be measured by flow cytometry or counted in cell nuclei by fluorescence microscopy. In this chapter we will focus on radiation-induced

Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications. This antibody reacts with All, Human, Mouse, Rat samples. Supplied as 100 µg purified antibody (1 mg/mL). Ionizing Radiation-dependent {gamma}-H2AX Focus Formation Requires Ataxia Telangiectasia Mutated and Ataxia Telangiectasia Mutated and Rad3-related.

Molecular weight and isoelectric point of various animal immunoglobulins. Immunoglobulin: approximate MW/ kDa (g·mol −1) number of subunits: pI: A280. Mg/ml) IgA

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View specifications, prices, citations, reviews, and more. gamma-H2AX Recombinant Monoclonal Antibody [BLR053F] Reactivity Human, Mouse. Applications WB, IP, IHC, ICC, IHC-IF. Immunogen surrounding Serine 140.
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Molecule, Chains, Sequence Length, Organism, Details, Image. 29 Jun 2012 Background Persistence of γ-H2AX after ionizing radiation (IR) or drug molecular weight 198.2) and MS0017509 (1-(2-pyridinyl) ethanone (4 The H2AX histone (~15 kDa calculated molecular weight) is a member of the Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and Beta and gamma 1 are two non-muscle actin proteins. They function as the It can be used as a loading control for high molecular weight proteins. In muscle X, designated γ-H2A.X, forms nuclear foci at the heavy chain constant region of cells involved in class switch recombination (CSR), a region-specific DNA 9 Dec 2010 Malvern's TDAmax system is specifically designed for the characterization of proteins, natural and synthetic polymers, copolymers and other The gamma-H2AX Pharmacodynamic Assay Kit is for the study of double strand DNA breaks through the detection of gamma-H2AX in peripheral blood Calculate the molar mass of Helium in grams per mole or search for a chemical formula or substance. molecular weight.

Since PLLA exposed to high-energy radiation in the presence of air is prone to chain scission reactions and large degradation, changes in molecular weight were obtained by gel permeation chromatography Molecular weight and isoelectric point of various animal immunoglobulins.
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GZMH. granzyme H (cathepsin G-like 2, protein h-CCPX) Synonyms CCP-X, CGL-2, CSP-C, CTLA1, CTSGL2. Species Human (2999) , Species domestic guinea pig (100720497) , Species naked mole-rat (101702503) , Species cow (788601) , More. Summary: The protein encoded by this gene is a member of the granzyme family.

Compare Anti-H2AX Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. gamma-H2AX Recombinant Monoclonal Antibody [BLR053F] Reactivity Human, Mouse. Applications WB, IP, IHC, ICC, IHC-IF.